Enzymes and Inhibitors
Medchemexpress LLC H-Glu(AMC)-OH | 98516-76-8 | MFCD00038085 | 95.0% | 304.30 g/mol | C15H16N2O5 | 25 MG
H-Glu(AMC)-OH is an amino acid derivative used as a fluorogenic peptide substrate to measure aminopeptidase A activity. It releases the fluorescent leaving group 7-amino-4-methylcoumarin (AMC) upon enzymatic cleavage, enabling sensitive fluorometric assays for biochemical research. The material is supplied as a powder for laboratory use.
- Fluorogenic substrate for aminopeptidase A activity assays.
- Releases 7-amino-4-methylcoumarin (AMC) upon enzymatic cleavage for sensitive fluorescence detection.
- Powder form suitable for preparation of assay solutions.
- Stable when stored as a powder at -20°C (up to 3 years) or 4°C (up to 2 years).
- In-solution stability: short-term storage at -80°C (months) or -20°C (weeks) depending on solvent.
- Molecular formula C15H16N2O5; molecular weight 304.30 g/mol.
Cayman Chemical Ac-DEVD-AMC 5MG
A fluorogenic substrate that can be cleaved specifically by caspase-3
STEMCELL Technologies CoV Protease Substrate-1 EDANS, Size: 100 Tests
FRET-based peptide substrate for detection of protease activity of coronaviruses
Medchemexpress LLC Recombinant canine HER2 (CD340) protein, C-terminal His-tag | >95.0% | 10 UG
Recombinant canine HER2 (CD340) protein representing the extracellular region (T23-T652), produced in HEK293 mammalian cells and supplied with a C-terminal His tag. The product is provided lyophilized for reconstitution and is intended for use in biochemical assays, antibody characterization, immunoassays, and binding studies requiring the canine ERBB2 ectodomain.
- Expression in HEK293 mammalian cells for proper folding and glycosylation.
- C-terminal His tag for purification and detection.
- Includes amino acid range T23-T652 for extracellular domain studies.
- High purity (>95.0%) as determined by reducing SDS-PAGE.
- Available in small pack sizes including 10 μg for low-volume experiments.
- Reconstitution recommended to ≥100 μg/mL; store at -20°C for long-term stability.
Enzo Life Sciences OMNICATHEPSIN fluorogenic substrate (50mg). CAS: 65147-22-0
Fluorogenic substrate cleaved by plasma kallikrein and kallikrein 8, but not plasmin; by papain; and by most cathepsins (cathepsins B, C/DPP-I, F, K/O2, L, L2/V, O, S, and X/Z). Ex.: 380 nm, Em.: 460 nm, although the following Ex/Em can also be used: 355,375/440,450. This substrate is useful for inhibitor screening and kinetic analysis. Also available: fluorogenic calibration standard AMC (BML-KI144). Alternative name: Z-FR-AMC, Z-Phe-Arg-AMC. Purity: ≥99%. Long Term Storage: -20°C.
Enzo Life Sciences Histone H3 dimethyl lysine-4 peptide (0.5mg)
Residues 1-21 from the N-terminal tail of human histone H3, dimethylated on the side-chain (ε-amino function) of Lys4. Useful as a substrate for histone lysine demethylases, for example LSD1. Purity: ≥95% (HPLC). Formulation: Lyophilized. Solubility: Soluble in Water. Long Term Storage: -20°C.
Medchemexpress LLC HY-107380A 10mg Medchemexpress, PGlu-3-methyl-His-Pro-NH2 (TFA) CAS: Purity:>98%
Medchemexpress, HY-107380A 10mg PGlu-3-methyl-His-Pro-NH2 (TFA) CAS: PGlu-3-methyl-His-Pro-NH2 TFA (A-42872 TFA), the modified thyrotropin-releasing hormone (TRH) peptide, enhances binding to pituitary TRH receptors and increases stimulation of thyroid-stimulating hormone (TSH) release from the pituitary. The in vitro permeability of PGlu-3-methyl-His-Pro-NH2 TFA through rat skin is increased in the presence of enhancers Ethanol and Cineole. Purity:>98% Medchemexpress has over 10000 novel life-science reagents, reference compounds, APIs and natural compounds for laboratory and scientific use. Other quantity can also be offered.
Abcam 2-Chloro-4-nitrophenyl-Beta-D-lactoside, 25MG
MW 497.8 Da, Purity >98%. Highly sensitive lactosidase, yellow coloured probe that releases the product 2-chloro-4-nitrophenol (CNP). Can be monitored spectrophotometrically at 405 nm. The rate of formation of 2-chloro-4-nitrophenol is directly proportional to the lactosidase activity in the sample. The pKa of CNP is approximately 5.5.
The product is subject to the following: Abcam Restricted Use Statement
Zymo Research Corporation 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-GAL) (5 ml) ^ '*'
Ready-to-use sterile 2% X-GAL solution that can be added directly to your agar plates. The X-GAL solution has been uniquely formulated to reduce false positives during blue-white screening and generates a rich blue color that is easily distinguishable from background.
Cayman Chemical Gly-Gly-AMC 25mg
A fluorogenic peptide substrate; used to assess the activity of bacterial proteases from P. aeruginosa and S. aureus; enzyme activity can be quantified by fluorescent detection of free AMC (also known as 7-amino-4-methylcoumarin), which is excited at 340-360 nm and emits at 440-460 nm
Abcam Bluo-Gal, Chromogenic beta-galactosidase substrate, 250MG
MW 374.2 g/mol, Purity >98%. Chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Designed as an alternative to X-Gal (ab144388) in blue-white selection of recombinant bacterial colonies with the lac+ phenotype, producing a darker blue color.
The product is subject to the following: Abcam Restricted Use Statement
Medchemexpress LLC Z-val-val-arg-AMC | 124485-41-2 | MFCD00238523 | 99.8% | 663.76 | C34H45N7O7 | 5 MG
Z-Val-Val-Arg-AMC is a fluorogenic peptide substrate used to measure cathepsin protease activity in biochemical and enzymatic assays. The peptide contains a benzyloxycarbonyl (Z) protecting group and a 7-amino-4-methylcoumarin (AMC) fluorescent leaving group; cleavage by cathepsins releases AMC and generates a measurable fluorescence signal. For research use only.
- Fluorogenic substrate for cathepsin activity assays.
- Sequence: {Z}-Val-Val-Arg-{AMC}.
- High purity (99.8%).
- Molecular weight 663.76.
- Chemical formula C34H45N7O7.
- Available in small mg-scale packages for assay development.
Enzo Life Sciences Cathepsin D & E substrate (fluorogenic) (1 mg)
Quenched fluorogenic substrate for cathepsin D (kcat/Km=1.09 x 10 M-1s-1) and cathepsin E (kcat/Km=1.56 x 10 M-1s-1). Mca fluorescence is quenched by the Dnp group until cleavage separates them. Activity of the two enzymes on this substrate can be distinguished by the use of Ascaris pepsin inhibitor, which inhibits cathepsin E, but not cathepsin D. This substrate is also cleaved by MMP-2, MMP-3, MMP-9, but not MMP-1 nor MMP-13. This substrate is useful for inhibitor screening and kinetic analysis. Ex.: 320-340 nm, Em.: 393-420 nm. Mca-Pro-Leu-OH (BML-P127) can be used to calibrate for Mca fluorescence. MW: 1756.0. Formulation: Trifluroacetate salt. Purity: ≥95% (HPLC). Sequence Mca-Gly-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Lys(Dnp)-D-Arg-NH2 [Mca= (7-methoxycoumarin-4-yl)acetyl; Dnp=dinitrophenyl]. Appearance: Yellow powder. Solubility: Soluble in DMSO (1mM) or 50% acetonitrile (10mg/ml). Long Term Storage: -20°C. Handling: Protect from light. Keep cool and dry.
Biotium NucView 530 Caspase-3 Substrate, 1 mM in DMSO, Trial Size
NucView Caspase-3 Substrates are novel fluorogenic substrates for real-time or endpoint detection of apoptosis in live cells. The non-toxic, no-wash substrates do not inhibit caspase-3 unlike other caspase substrates or FLICA assays permitting multiday analysis. Consisting of a high-affinity DNA dye linked to a DEVD caspase-3 substrate moiety, the cell permeable non-fluorescent substrate is cleaved by caspase-3 in apoptotic cells to emit fluorescence, allowing detection of both caspase-3 activity and apopototic nuclear morphology. Suitable for flow cytometry, fluorescence microscopy, or live cell imaging systems, staining is compatible with fixation and permeabilization for immunostaining. NucView 488 is validated in over 100 cell types and 200 publications. Available with blue (NucView 405), green (NucView 488), or red (NucView 530) fluorescence, in DMSO or PBS for DMSO-sensitive cells, and as kits with apoptosis or necrosis probes. NucView technology is covered by U.S. patents.
Bioworld 4-Methylumbelliferyl-A-L-Iduronide, 1 mg
Substrate for alpha-L-IduronidaseSpecification: * Slightly soluble in water This product is sold for laboratory research use onlyReference:Use of 4-methylumbelliferryl-alpha-L-iduronide and 4-trifluoromethylumbelliferryl-alpha-L-iduronide for detecting alpha-L-iduronidase deficiencies in human tissue and for rapid prenatal diagnosis of Hurler disease. Vopr Med Khim. 1991 Jan-Feb; 37 (1):74-7.